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bio rad micro bio spin 6 centrifugal gel filtration columns  (Bio-Rad)


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    Structured Review

    Bio-Rad bio rad micro bio spin 6 centrifugal gel filtration columns
    Bio Rad Micro Bio Spin 6 Centrifugal Gel Filtration Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+micro+bio+spin+6+centrifugal+gel+filtration+columns/Micro+Bio-Spin+6/pm41237214-190-38-48
    Average 96 stars, based on 875 article reviews
    bio rad micro bio spin 6 centrifugal gel filtration columns - by Bioz Stars, 2026-09
    96/100 stars

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    Filtration:

    Article Title: Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O-O bond formation.
    Article Snippet: .. For cryo-EM, a thawed sample was exchanged into cryo-EM buffer (0.5 M betaine, 50 mM HEPES-NaOH [pH 6.8], 20 mM CaCl2, 5 mM MgCl2, and 0.02% [w/v] β-DDM) at 4 °C using Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 × g. Note that, based on previous results (15), we did not think the 50 mM Cl-1 concentration would affect the binding of Cl1, so it was maintained in the cryo-EM buffer. .. The sample was concentrated using Amicon Ultra 0.5-mL 100-kDa centrifugal filters (EMD Millipore, Billerica) to ~2 mg of Chl mL-1 and kept in complete darkness at 4 °C until plunge freezing.

    Article Title: Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels
    Article Snippet: .. For cryo-EM analyses, thawed samples were exchanged into cryo-EM buffer (0.5 M betaine, 50 mM MES-NaOH [pH 6.8], 20 mM CaCl 2 , 5 mM MgCl 2 , and 0.02% [w/v] β-DDM) at 4 °C by passage through Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories) at 50 g . Samples were then concentrated to ∼2 mg of Chl/ml with Amicon Ultra 0.5-mL 100-kDa centrifugal filter devices (EMD Millipore) and kept at ∼4 °C until being loaded onto cryo-EM grids. .. Holey carbon QUANTIFOIL R 2/1 Cu 300-mesh EM grids (purchased from Electron Microscopy Sciences) were glow discharged for 30 s at 25 mA.

    Article Title: Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O–O bond formation
    Article Snippet: .. For cryo-EM, a thawed sample was exchanged into cryo-EM buffer (0.5 M betaine, 50 mM HEPES-NaOH [pH 6.8], 20 mM CaCl 2 , 5 mM MgCl 2 , and 0.02% [w/v] β-DDM) at 4 °C using Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 g . Note that, based on previous results , we did not think the 50 mM Cl - concentration would affect the binding of Cl1, so it was maintained in the cryo-EM buffer. .. The sample was concentrated using Amicon Ultra 0.5-mL 100-kDa centrifugal filters (EMD Millipore, Billerica) to ∼2 mg of Chl mL −1 and kept in complete darkness at 4 °C until plunge freezing.

    Article Title: High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803.
    Article Snippet: .. To prepare samples for cryo-EM analysis, aliquots (50 μg of Chl a) were exchanged into cryo-EM buffer (0.5 M betaine, 50 mMMES-NaOH [pH 6.8], 20 mM CaCl2, 5 mM MgCl2, 0.02% [wt/vol] β-DM) by passage through Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 × g and then concentrated to ∼2 mg of Chl/mL with Amicon Ultra 0.5-mL 100-kDa centrifugal filter devices (EMD Millipore, Billerica). .. Samples were maintained at ∼4 °C until being applied to cryo-EM grids.

    Article Title: Structure of the D1-Val185Asn mutated photosystem II complex with slow O-O bond formation reveals changes in the Cl1 water channel.
    Article Snippet: .. For cryo- EM usage, the sample was thawed and exchanged into cryo- EM buffer [0.5 M betaine, 50 mM HEPES- NaOH (pH 6.8), 20 mM CaCl2, 5 mM MgCl2, and 0.02% (w/v) β- DDM] at 4 °C using Bio- Rad Micro Bio- Spin 6 centrifugal gel filtration columns (Bio- Rad Laboratories, Hercules) at 50×g before being concentrated using Amicon Ultra 0.5- mL 100- kDa centrifugal filters (EMD Millipore, Billerica) to ~2 mg of Chl mL−1. ..

    Concentration Assay:

    Article Title: Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O-O bond formation.
    Article Snippet: .. For cryo-EM, a thawed sample was exchanged into cryo-EM buffer (0.5 M betaine, 50 mM HEPES-NaOH [pH 6.8], 20 mM CaCl2, 5 mM MgCl2, and 0.02% [w/v] β-DDM) at 4 °C using Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 × g. Note that, based on previous results (15), we did not think the 50 mM Cl-1 concentration would affect the binding of Cl1, so it was maintained in the cryo-EM buffer. .. The sample was concentrated using Amicon Ultra 0.5-mL 100-kDa centrifugal filters (EMD Millipore, Billerica) to ~2 mg of Chl mL-1 and kept in complete darkness at 4 °C until plunge freezing.

    Article Title: Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O–O bond formation
    Article Snippet: .. For cryo-EM, a thawed sample was exchanged into cryo-EM buffer (0.5 M betaine, 50 mM HEPES-NaOH [pH 6.8], 20 mM CaCl 2 , 5 mM MgCl 2 , and 0.02% [w/v] β-DDM) at 4 °C using Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 g . Note that, based on previous results , we did not think the 50 mM Cl - concentration would affect the binding of Cl1, so it was maintained in the cryo-EM buffer. .. The sample was concentrated using Amicon Ultra 0.5-mL 100-kDa centrifugal filters (EMD Millipore, Billerica) to ∼2 mg of Chl mL −1 and kept in complete darkness at 4 °C until plunge freezing.

    Binding Assay:

    Article Title: Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O-O bond formation.
    Article Snippet: .. For cryo-EM, a thawed sample was exchanged into cryo-EM buffer (0.5 M betaine, 50 mM HEPES-NaOH [pH 6.8], 20 mM CaCl2, 5 mM MgCl2, and 0.02% [w/v] β-DDM) at 4 °C using Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 × g. Note that, based on previous results (15), we did not think the 50 mM Cl-1 concentration would affect the binding of Cl1, so it was maintained in the cryo-EM buffer. .. The sample was concentrated using Amicon Ultra 0.5-mL 100-kDa centrifugal filters (EMD Millipore, Billerica) to ~2 mg of Chl mL-1 and kept in complete darkness at 4 °C until plunge freezing.

    Article Title: Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O–O bond formation
    Article Snippet: .. For cryo-EM, a thawed sample was exchanged into cryo-EM buffer (0.5 M betaine, 50 mM HEPES-NaOH [pH 6.8], 20 mM CaCl 2 , 5 mM MgCl 2 , and 0.02% [w/v] β-DDM) at 4 °C using Bio-Rad Micro Bio-Spin 6 centrifugal gel filtration columns (Bio-Rad Laboratories, Hercules) at 50 g . Note that, based on previous results , we did not think the 50 mM Cl - concentration would affect the binding of Cl1, so it was maintained in the cryo-EM buffer. .. The sample was concentrated using Amicon Ultra 0.5-mL 100-kDa centrifugal filters (EMD Millipore, Billerica) to ∼2 mg of Chl mL −1 and kept in complete darkness at 4 °C until plunge freezing.

    Cryo-EM Sample Prep:

    Article Title: Structure of the D1-Val185Asn mutated photosystem II complex with slow O-O bond formation reveals changes in the Cl1 water channel.
    Article Snippet: .. For cryo- EM usage, the sample was thawed and exchanged into cryo- EM buffer [0.5 M betaine, 50 mM HEPES- NaOH (pH 6.8), 20 mM CaCl2, 5 mM MgCl2, and 0.02% (w/v) β- DDM] at 4 °C using Bio- Rad Micro Bio- Spin 6 centrifugal gel filtration columns (Bio- Rad Laboratories, Hercules) at 50×g before being concentrated using Amicon Ultra 0.5- mL 100- kDa centrifugal filters (EMD Millipore, Billerica) to ~2 mg of Chl mL−1. ..



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